Journal: Nature Communications

Article Title: An immune checkpoint score system for prognostic evaluation and adjuvant chemotherapy selection in gastric cancer

doi: 10.1038/s41467-020-20260-7

Figure Lengend Snippet: Multi-colour immunofluorescence staining of anti-CKpan and CD45 antibodies with CD155, NECTIN2, CEACAM1, HMGB1, SIGLEC6 or CD44 antibodies. Scale bar = 200 μm.

Article Snippet: Multiple immunofluorescence staining was performed to identify the expression of six indicators (NECTIN2 (95333S, CST, 1 : 100), CEACAM1 (44464S, CST, 1 : 200), HMGB1 (ab18256, Abcam, 1 : 800), SIGLEC6 (ab38581, Abcam, 1 : 100), CD44 (3570S, CST, 1 : 50), CD155 (81254S, CST, 1 : 200)), CD45 (ab10558, Abcam, 1 : 100) and panCK (ab7753, Abcam, 1 : 100) in 135 types of GC tissues.

Techniques: Immunofluorescence, Staining

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: COM902, a novel therapeutic antibody targeting TIGIT augments anti-tumor T cell function in combination with PVRIG or PD-1 pathway blockade

doi: 10.1007/s00262-021-02921-8

Figure Lengend Snippet: COM902 does not induce ADCC or CDC activity. a ADCC activity assessment for COM902. b CDC activity assessment for COM902. Campath (anti-CD52) hIgG1 was used as a positive control. Representative data from n ≥ 2 is shown for each graph

Article Snippet: For blocking experiments, the cell lines described above were pre-incubated with COM902 or hIgG4 isotype for 30 min. Recombinant hPVR-mIgG2a-Fc (hPVR-Fc) and cynomolgus PVR-hIgG1-Fc-biotin (cPVR-Fc) produced internally, or mouse PVR-hIgG1-Fc (mPVR-Fc) (Sino Biological) were added to the cells for 1 h and detected with fluorescently labeled secondary antibodies.

Techniques: Activity Assay, Positive Control